06322980 is referenced by 43 patents and cites 11 patents.

Methods and compositions are provided for detecting single nucleotide polymorphisms using a pair of oligonucleotides, a primer and a snp detection sequence, where the snp detection sequence hybridizes to the target DNA downstream from the primer and in the direction of primer extension. The snp detection sequence is characterized by having a nucleotide complementary to the snp and adjacent nucleotide complementary to adjacent nucleotides in the target and an electophoretic tag bonded to the 5′-nucleotide. The pair of oligonucleotides is combined with the target DNA under primer extension conditions, where the polymerase has 5′-3′ exonuclease activity. When the snp is present, the electophoretic tag is released from the snp detection sequence, and can be detected by electrophoresis as indicative of the presence of the snp in the target DNA.

Title
Single nucleotide detection using degradation of a fluorescent sequence
Application Number
9/303029
Publication Number
6322980 (B1)
Application Date
April 30, 1999
Publication Date
November 27, 2001
Inventor
Sharat Singh
San Jose
CA, US
Assignee
Aclara Biosciences
CA, US
IPC
C12Q 1/68
View Original Source