06306273 is referenced by 117 patents and cites 20 patents.

A method is disclosed for controlling the direction and transport of a material on a microfluidic device formed from an acrylic polymer, by electrokinetic flow of a fluid containing the material. The method comprises providing an electrokinesis buffer containing a charged hydrophilic polymer, wherein charges on the polymer are randomly distributed. The method has applications for improved transport of proteins and transport of materials comprising differentially charged chemical species. A preferred embodiment of the present invention comprises use of the disclosed method for electrokinetic separations of a mixture of polypeptides having both positive and negative charges. Another preferred embodiment concerns use of the disclosed method for performing an assay involving mixing of two or more reagents, wherein a first reagent comprises an enzyme and a second reagent comprises species having an opposite charge to the enzyme.

Title
Methods and compositions for conducting processes in microfluidic devices
Application Number
9/291169
Publication Number
6306273 (B1)
Application Date
April 13, 1999
Publication Date
October 23, 2001
Inventor
Sharat Singh
San Jose
CA, US
Irene Visser
San Ramon
CA, US
Ann K Wainright
Cupertino
CA, US
Agent
Perkins Coie
US
Assignee
Aclara BioSciences
CA, US
IPC
G01N 27/26
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