06280986 is referenced by 62 patents and cites 3 patents.

Recombinant bacteria are disclosed which are transformed with heterologous DNA coding for alcohol dehydrogenase (adh) and pyruvate decarboxylase (pdc), and which are effective for use in the production of ethanol, but which do not require the presence of antibiotics in the culture medium to maintain genetic stability and high ethanol productivity. These recombinant bacteria are produced using mutant host strains which are substantially deficient in the ability to fermentatively reduce pyruvate. When grown in an anaerobic environment, the recombinant pyruvate mutants transformed with the adh and pdc genes are genetically stable, maintaining the inserted genes and ethanol productivity even in the absence of antibiotics.

Title
Stabilization of pet operon plasmids and ethanol production in bacterial strains lacking lactate dehydrogenase and pyruvate formate lyase activities
Application Number
9/201449
Publication Number
6280986 (B1)
Application Date
November 30, 1998
Publication Date
August 28, 2001
Inventor
Rodney J Bothast
East Peoria
IL, US
Bruce S Dien
Peoria
IL, US
Herbert A Wyckoff
Roscoe
IL, US
Robert B Hespell
late of Peoria
IL, US
Agent
John D Fado
US
Randall E Deck
US
M Howard Silverstein
US
Assignee
The United States of America represented by the Secretary of Agriculture
US
IPC
C12P 7/06
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