06265150 is referenced by 182 patents and cites 4 patents.

Peripheral blood leucocytes incubated with a semi-synthetic phage antibody library and fluorochrome-labeled CD3 and CD20 antibodies were used to isolate human single chain Fv antibodies specific for subsets of blood leucocytes by flow cytometry. Isolated phage antibodies showed exclusive binding to the subpopulation used for selection or displayed additional binding to a restricted population of other cells in the mixture. At least two phage antibodies appeared to display hithereto unknown staining patterns of B lineage cells. This approach provides a subtractive procedure to rapidly obtain human antibodies against known and novel surface antigens in their native configuration, expressed on phenotypically defined subpopulations of cells. Importantly, this approach does not depend on immunization procedures or the necessity to repeatedly construct phage antibody libraries.

Title
Phage antibodies
Application Number
9/85072
Publication Number
6265150 (B1)
Application Date
May 26, 1998
Publication Date
July 24, 2001
Inventor
Ton Logtenberg
Utrecht
US
Leon WMM Terstappen
Huntingdon Valley
PA, US
Agent
Hoffman & Baron
US
Agent
Irving N Feit
US
Assignee
Crucell Holland
US
Becton Dickinson & Company
NJ, US
IPC
C12Q 1/70
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