06174670 is referenced by 363 patents and cites 86 patents.

Methods of monitoring hybridization during polymerase chain reaction are disclosed. These methods are achieved with rapid thermal cycling and use of double stranded DNA dyes or specific hybridization probes. A fluorescence resonance energy transfer pair comprises fluorescein and Cy5 or Cy5.5. Methods for quantitating amplified DNA and determining its purity are carried out by analysis of melting and reannealing curves.

Title
Monitoring amplification of DNA during PCR
Application Number
8/869276
Publication Number
6174670 (B1)
Application Date
June 4, 1997
Publication Date
January 16, 2001
Inventor
Randy P Rasmussen
Salt Lake City
UT, US
Kirk M Ririe
Idaho Falls
ID, US
Carl T Wittwer
Salt Lake City
UT, US
Agent
Barnes & Thornburg
US
Assignee
University of Utah Research Foundation
UT, US
IPC
C12Q 1/68
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