06162963 is referenced by 291 patents and cites 17 patents.

The subject invention provides non-human mammalian hosts characterized by inactivated endogenous Ig loci and functional human Ig loci for response to an immunogen to produce human antibodies or analogs thereof. The hosts are produced by multiple genetic modifications of embryonic cells in conjunction with breeding. Different strategies are employed for recombination of the human loci randomly or at analogous host loci. Chimeric and transgenic mammals, particularly mice, are provided, having stably integrated large, xenogeneic DNA segments. The segments are introduced by fusion with yeast spheroplasts comprising yeast artificial chromosomes (YACs) which include the xenogeneic DNA segments and a selective marker such as HPRT, and embryonic stem cells.

Title
Generation of Xenogenetic antibodies
Application Number
466008
Publication Number
6162963
Application Date
June 5, 1995
Publication Date
December 19, 2000
Inventor
Daniel J Capon
Hillsborough
CA, US
Daniel G Brenner
Redwood City
CA, US
Sue Klapholz
Stanford
CA, US
Aya Jakobovits
Menlo Park
CA, US
Raju Kucherlapati
Darien
CT, US
Agent
Fish & Neave
Agent
Jane T Gunnison Esq
James F Haley Jr Esq
Assignee
Abgenix
CA, US
IPC
C12N 15/00
View Original Source