06117679 is referenced by 241 patents and cites 78 patents.

A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.

Title
Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
Application Number
537874
Publication Number
6117679
Application Date
March 25, 1996
Publication Date
September 12, 2000
Inventor
Willem P C Stemmer
Los Gatos
CA, US
Agent
Joe Liebeschuetz
Norman J Kruse
Assignee
Maxygen
CA, US
IPC
C07H 21/04
C07H 21/02
C12Q 1/68
C12N 15/00
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