05928914 is referenced by 50 patents and cites 5 patents.

Methods and compositions for transforming cells, resulting in efficient and stable site-specific integration of transgenes, are disclosed. Transformation is achieved by introducing into a cell an acceptor vector, preferably a retroviral vector, which integrates into the genome of the cell. The acceptor vector comprises two incompatible lox sequences, L1 and L2. A donor vector is then introduced into the cell comprising a transgene flanked by the same L1 and L2 sequences. Stable gene transfer is initiated by contacting the lox L1 and L2 sequences with Cre recombinase.

Title
Methods and compositions for transforming cells
Application Number
664084
Publication Number
5928914
Application Date
November 5, 1996
Publication Date
July 27, 1999
Inventor
Ken Julian Takekoshi
Bethesda
MD, US
Karen Westerman
Reading
MA, US
Eric Bouhassira
Hastings on Hudson
NY, US
Philippe Leboulch
Cambridge
MA, US
Agent
Lahive & Cockfield
Agent
Jane E Remillard Esq
Assignee
Massachusetts Institute of Technology
MA, US
Albert Einstein College of Medicine of Yeshiva University a Division of Yeshiva University
NY, US
IPC
C07H 21/04
C12P 21/02
C12N 15/64
C12N 15/52
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