05922350 is referenced by 35 patents and cites 33 patents.

Dehydrated liposomes are prepared by drying liposome preparations under reduced pressure in the presence of one or more protective sugars, e.g., the disaccharides trehalose and sucrose. Preferably, the protective sugars are present at both the inside and outside surfaces of the liposome membranes. Freezing of the liposome preparation prior to dehydration is optional. Alternatively, the protective sugar can be omitted if: (1) the liposomes are of the type which have multiple lipid layers; (2) the dehydration is done without prior freezing; and (3) the dehydration is performed to an end point which results in sufficient water being left in the preparation (e.g., at least 12 moles water/mole lipid) so that the integrity of a substantial portion of the multiple lipid layers is retained upon rehydration. Concentration gradients capable of generating transmembrane potentials can be created across the liposome membranes either before or after dehydration, and the transmembrane potentials resulting from these gradients can be used to load charged materials, e.g., drugs, into the liposomes.

Title
Methods of dehydrating, storing and rehydrating liposomes
Application Number
749161
Publication Number
5922350
Application Date
June 16, 1997
Publication Date
July 13, 1999
Inventor
Regina L Jablonski
Trenton
NJ, US
Hugh P Schieren
Yardley
PA, US
Thomas D Madden
Vancouver
CA
Michael J Hope
Vancouver
CA
Richard S Ginsberg
Monroe
NJ, US
Michael W Fountain
Griggstown
NJ, US
Marcel B Bally
Vancouver
CA
Pieter R Cullis
Vancouver
CA
Andrew S Janoff
Yardley
PA, US
Agent
Rosanne Goodman
Kenneth B Rubin
Assignee
The Liposome Company
NJ, US
IPC
B01J 13/04
B01J 13/02
A61K 9/127
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