05897842 is referenced by 64 patents and cites 11 patents.

An apparatus and method for thermally cycling a reaction mixture in a reaction vessel to expose the mixture to the varying temperatures necessary to, for example, achieve PCR amplification or the preparation of sequencing fragments using a cycle sequencing operation makes use of flow-through reaction vessels, such as capillary tubes, for the preparation and thermal cycling of reaction mixtures. In order to prevent loss of the reaction mixture from the vessels during heating, the thermal cycling apparatus of the invention provides means for sealing the proximal and distal end of each reaction vessel. The proximal ends can be sealed by coupling to a pump which permits movement of the samples within the reaction vessels. As to the distal ends, the reaction vessels can be sealed by pressing the distal end of each vessel against a sealing element with a conformable surface, or by immersing the distal end of each vessel in the reservoir of liquid, preferably of an oil, that is not miscible with the reaction mixture.

Title
Method and apparatus for thermal cycling and for automated sample preparation with thermal cycling
Application Number
684498
Publication Number
5897842
Application Date
August 27, 1996
Publication Date
April 27, 1999
Inventor
Henryk Zaleski
Niagara Falls
CA
Alexandre M Izmailov
Toronto
CA
Paul Waterhouse
Copetown
CA
John Renfrew
Burlington
CA
James Leushner
North York
CA
James M Dunn
Scarborough
CA
Agent
Oppedahl & Larson
Assignee
Visible Genetics
CA
IPC
C12M 3/02
B01L 9/00
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