05859307 is referenced by 3 patents and cites 1 patents.

Immunodeficient animals are generated by introducing a mutation in RAG-1 into the germline of the animals via gene targeting in embryonic stem cells. The production of mutant RAG-1 deficient mice is detailed. RAG-1 deficient mice have no mature B and T lymphocytes. The arrest of B and T cell differentiation occurs at an early stage and correlates with the inability to perform V(D)J recombination. To date, these mice do not have mature B and T lymphocytes, nor do they express immunoglobulin or T cell receptors. The same strategy can be applied to the generation of other RAG-1 deficient animals, such as rabbits, rats, and pigs, using known techniques. These animals are all useful for the same general purposes as the scid mice, for example, cultivation of human lymphocytes for expression of human immunoglobulin. Other uses include the establishment of continuous lymphoid cell lines and, by crossbreeding with other lines of animals, the establishment of animals developing tumors for use in studying tumor cell developments and treatments.

Title
Mutant RAG-1 deficient animals having no mature B and T lymphocytes
Application Number
830831
Publication Number
5859307
Application Date
June 7, 1994
Publication Date
January 12, 1999
Inventor
Virginia Papaioannou
Lexington
MA, US
Randall S Johnson
Boston
MA, US
Susumu Tonegawa
Newton
MA, US
Peter Mombaerts
Cambridge
MA, US
Agent
Arnall Golden & Gregory
Assignee
Dana Farber Cancer Institute & Tufts University
MA, US
Massachusetts Institute of Technology
MA, US
IPC
C12N 15/06
C12N 5/06
G01N 31/00
A61K 49/00
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