05856092 is referenced by 201 patents and cites 21 patents.

A method for detecting a specific polynucleotide sequence in a material is disclosed. The method includes exposing said material to an oligonucleotide primer having a sequence complementary to part of said specific polynucleotide sequence wherein said primer binds to part of said polynucleotide sequence when present in said material. Primer bound to the polynucleotide sequence is extended wherein any extended primer includes a detectable element and/or a separation element. Any extended primer is then separated into a fraction wherein said fraction does not have detectable element not included in said extended primer. One then determines whether any extended primer is present in said fraction by assaying said fraction for said extended primer wherein the presence of said extended primer is indicative of the presence of the specific polynucleotide sequence in said material and the absence of said extended primer is indicative of the presence of the specific polynucleotide sequences in said material and the absence of said extended primer in said fraction is indicative of the absence of the specific polynucleotide sequence in said material.

Title
Detection of a nucleic acid sequence or a change therein
Application Number
744766
Publication Number
5856092
Application Date
April 27, 1995
Publication Date
January 5, 1999
Inventor
Terence Patrick Walsh
Acacia Ridge
AU
Peter Timms
East Ipswitch
AU
James Langham Dale
Moggill
AU
Agent
Marshall O Toole Gerstein Murray & Borun
Assignee
Geneco
AU
IPC
C12N 15/00
C07H 21/00
C12P 19/34
C12Q 1/68
View Original Source