05846719 is referenced by 214 patents and cites 9 patents.

The invention provides a method of tracking, identifying, and/or sorting classes or subpopulations of molecules by the use of oligonucleotide tags. Oligonucleotide tags of the invention comprise oligonucleotides selected from a minimally cross-hybridizing set. Preferably, such oligonucleotides each consist of a plurality of subunits 3 to 9 nucleotides in length. A subunit of a minimally cross-hybridizing set forms a duplex or triplex having two or more mismatches with the complement of any other subunit of the same set. The number of oligonucleotide tags available in a particular embodiment depends on the number of subunits per tag and on the length of the subunit. An important aspect of the invention is the use of the oligonucleotide tags for sorting polynucleotides by specifically hybridizing tags attached to the polynucleotides to their complements on solid phase supports. This embodiment provides a readily automated system for manipulating and sorting polynucleotides, particularly useful in large-scale parallel operations, such as large-scale DNA sequencing, mRNA fingerprinting, and the like, wherein many target polynucleotides or many segments of a single target polynucleotide are sequenced simultaneously.

Title
Oligonucleotide tags for sorting and identification
Application Number
322348
Publication Number
5846719
Application Date
June 6, 1996
Publication Date
December 8, 1998
Inventor
Stephen C Macevicz
Cupertino
CA, US
Glenn Albrecht
Redwood City
CA, US
Sydney Brenner
Cambridge
GB
Agent
Stephen C Macevicz
Assignee
Lynx Therapeutics
CA, US
IPC
C07H 21/04
C07H 21/00
C12Q 1/68
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