05776748 is referenced by 237 patents and cites 9 patents.

The disclosed invention is a device for adhering cells in a specific and predetermined position. The device comprises a plate defining a surface and a plurality of cytophilic islands that adhere cells which are isolated by cytophobic regions to which cells do not adhere and further is contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM). Further, the cytophobic regions are wide enough such that less than 10 percent of the cells adhered to the cytophilic islands are allowed to form bridges across the cytophobic regions and contact each other. The device is used in a method for culturing cells on a surface or in a medium and also for performing cytometry. Furthermore, the device is used in immobilization of cells at a surface and for controlling the shape of a cell.

Title
Method of formation of microstamped patterns on plates for adhesion of cells and other biological materials, devices and uses therefor
Application Number
131838
Publication Number
5776748
Application Date
June 6, 1996
Publication Date
July 7, 1998
Inventor
Gregory N Stephanopoulos
Winchester
MA, US
Daniel I C Wang
Belmont
MA, US
Gabriel P Lopez
Albuquerque
NM, US
Donald E Ingber
Boston
MA, US
George M Whitesides
Newton
MA, US
Amit Kumar
Sacramento
CA, US
Rahul Singhvi
Cambridge
MA, US
Agent
Wolf Greenfield & Sacks P C
Assignee
Children s Medical Center Corporation
MA, US
Massachusetts Institute of Technology
MA, US
President and Fellows of Harvard College
MA, US
IPC
C12N 11/08
C12N 11/14
B32B 31/00
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