05773267 is referenced by 30 patents.

The present invention provides a conditional shuttle phasmid constructed by inserting a cosmid into a non-essential region of the D29 mycobacteriophage which is capable of introducing DNA of interest into the chromosome of mycobacteria, especially M. tuberculosis complex organisms and other slow growing mycobacteria. The present invention provides a recombinant mycobacterium which expresses a DNA of interest incorporated into its chromosome by a conditional shuttle plasmid containing the DNA of interest. The present invention further provides a mycobacterial auxotrophic mutant and method of generating auxotrophic mutants. Finally, the present invention provides a method of inactivating a mycobacterial virulence gene.

Title
D29 shuttle phasmids and uses thereof
Application Number
833431
Publication Number
5773267
Application Date
March 7, 1996
Publication Date
June 30, 1998
Inventor
Graham F Hatfull
Pittsburgh
PA, US
William R Jacobs
City Island
NY, US
Agent
Amster Rothstein & Ebenstein
Assignee
University of Pittsburgh
PA, US
Albert Einstein College of Medicine of Yeshiva University a Division of Yeshiva University
NY, US
IPC
C12N 15/74
C12N 15/63
C12N 15/11
C12N 1/21
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