05770460 is referenced by 76 patents and cites 34 patents.

A particularly efficient design for a nonbibulous lateral flow one step assay for an analyte in a biological sample is disclosed. In the improved device of the invention, three zones which are in nonbibulous lateral flow contact are employed: a sample receiving zone, a labeling zone, and a capture zone. The sample containing analyte is carried through the labeling zone and interacts with an assay label comprising visible moieties, preferably particles, which are coupled to specific binding reagent for analyte or to a competitor with analyte for a capture reagent. The flow continues into the capture zone where the visible moieties to which analyte or competitor are coupled are captured. Excess fluid is absorbed into an absorbent zone in contact with the capture zone. A positive result is obtained by visualizing the visible moieties in the capture zone. An additional improvement comprises obtaining the nonbibulous lateral flow by converting a bibulous support to a nonbibulous support through coating with a blocking agent, such a methylated BSA. Control label comprising visible moieties (preferably, visually distinguishable from those of the assay label) may also be included in the labeling zone and captured in a separate control portion of the capture zone to verify that the flow of liquid is as expected.

Title
One-step lateral flow nonbibulous assay
Application Number
639967
Publication Number
5770460
Application Date
June 7, 1995
Publication Date
June 23, 1998
Inventor
Keren Goins
San Diego
CA, US
Allan D Pronovost
San Diego
CA, US
Catherine Pawlak
Cardiff
CA, US
Agent
Morrison & Foerster
Assignee
Quidel Corporation
CA, US
IPC
G01N 33/532
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