05756291 is referenced by 152 patents and cites 4 patents.

A method for identifying oligomer sequences, optionally comprising modified base, which specifically bind target molecules such as serum proteins, kinins, eicosanoids and extracellular proteins is described. The method is used to generate aptamers that bind to serum Factor X, PDGF, FGF, ICAM, VCAM, E-selectin, thrombin, bradykinin, PGF2 and cell surface molecules. The technique involves complexation of the target molecule with a mixture of oligonucleotides containing random sequences and sequences which serve as primer for PCR under conditions wherein a complex is formed with the specifically binding sequences, but not with the other members of the oligonucleotide mixture. The complex is then separated from uncomplexed oligonucleotides and the complexed members of the oligonucleotide mixture are recovered from the separated complex using the polymerase chain reaction. The recovered oligonucleotides may be sequenced, and successive rounds of selection using complexation, separation, amplification and recovery can be employed. The oligonucleotides can be used for therapeutic and diagnostic purposes and for generating secondary aptamers.

Title
Aptamers specific for biomolecules and methods of making
Application Number
934387
Publication Number
5756291
Application Date
June 7, 1995
Publication Date
May 26, 1998
Inventor
John J Toole
Burlingame
CA, US
Eric Vermaas
Oakland
CA, US
Lawrence Leung
Hillsborough
CA, US
John Latham
Palo Alto
CA, US
Glenn Albrecht
Redwood City
CA, US
Linda Griffin
Atherton
CA, US
Agent
Mark L Bosse
Assignee
Gilead Sciences
CA, US
IPC
C07H 21/02
C07H 21/04
C07K 1/14
C12Q 1/68
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