05750341 is referenced by 173 patents and cites 10 patents.

Method and compositions are provided for analyzing nucleic acid sequences based on repeated cycles of duplex extension along a single stranded template. Preferably, such extension starts from a duplex formed between an initializing oligonucleotide and the template. The initializing oligonucleotide is extended in an initial extension cycle by ligating an oligonucleotide probe to its end to form an extended duplex. The extended duplex is then repeatedly extended by subsequent cycles of ligation. During each cycle, the identity of one or more nucleotides in the template is determined by a label on, or associated with, a successfully ligated oligonucleotide probe. Preferably, the oligonucleotide probe has a blocking moiety, e.g. a chain-terminating nucleotide, in a terminal position so that only a single extension of the extended duplex takes place in a single cycle. The duplex is further extended in subsequent cycles by removing the blocking moiety and regenerating an extendable terminus. The invention provides a method of sequencing nucleic acids which obviates electrophoretic separation of similarly sized DNA fragments, and which eliminates the difficulties associated with the detection and analysis of spacially overlapping bands of DNA fragments in a gel or like medium. The invention also obviates the need to generate DNA fragments from long single stranded templates with a DNA polymerase.

Title
DNA sequencing by parallel oligonucleotide extensions
Application Number
8/424663
Publication Number
5750341
Application Date
April 17, 1995
Publication Date
May 12, 1998
Inventor
Stephen C Macevicz
Cupertino
CA, US
Agent
Vincent M Dehlinger & Associates Powers
Stephen C Macevicz
Assignee
Lynx Therapeutics
CA, US
IPC
C12P 19/34
C07H 21/04
C07H 21/02
C12Q 1/68
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