05732150 is referenced by 71 patents and cites 23 patents.

A method and system for segmenting images of samples stained with two, three, or more stains accurately and reliably into multiple single-color images which represent the mass density of the stain at each position in the sample. The system includes a computer-controlled microscope and CCD camera, and at least one computer which includes data storage media for storing image data and which runs software for controlling image acquisition and analysis. To analyze tissue stained with N stains, images must be obtained at N different wavelengths, from multiply stained and singly stained samples. Relative stain mass densities are calculated with the use of the ratio of absorptivities measured at different wavelengths. The theoretical and practical basis for implementing the method with two, three, or more stains is presented. A method for selecting the wavelengths which provide optimal image accuracy and resolution is also provided.

Title
Method and system for multiple wavelength microscopy image analysis
Application Number
8/530435
Publication Number
5732150
Application Date
September 19, 1995
Publication Date
March 24, 1998
Inventor
Dennis L Parker
Centerville
UT, US
Elizabeth H Hammond
Salt Lake City
UT, US
Ruixia Zhou
Salt Lake City
UT, US
Agent
Mark G Sandbaken
Eleanor V Goodall
Assignee
IHC Health Services
UT, US
IPC
G06K 9/00
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