05725831 is referenced by 70 patents and cites 48 patents.

An apparatus for containing a liquid biological sample and for performing a biological process thereon comprises a sample area for receiving the sample, at least one reaction area in fluid communication with the sample area, a pneumatic area in pneumatic communication with the reaction area and the sample area, and a pneumatic port in the pneumatic area for connection of the apparatus to a pneumatic aspiration/dispensing pipette. The pneumatic aspiration/dispensing pipette provides for controlled flow of the liquid biological sample between the sample area and the reaction area. To reduce evaporative loss of the sample from the apparatus, a sample tower is provided in fluid communication with the sample area. A similar tower may be provided at the pneumatic port to reduce evaporative loss through the pneumatic area. A restricted orifice between the sample tower and the sample area holds the liquid sample in place during removal of the sample from the apparatus, counteracting the tendency of the liquid bolus to flow back toward the reaction area due to capillary forces. Also disclosed is a novel sealing arrangement which provides an effective pneumatic seal between the pneumatic port and a pneumatic aspiration/dispensing pipette without requiring the apparatus to include a separate "O" ring or other type of discrete sealing device.

Title
Nucleic acid amplification apparatus
Application Number
213304
Publication Number
5725831
Application Date
March 24, 1995
Publication Date
March 10, 1998
Inventor
Raymond F Cracauer
Plymouth
MN, US
Peter A Bourdelle
Glen Rock
PA, US
Allen S Reichler
Owings Mills
MD, US
Agent
David W Highet Esq
Assignee
Becton Dickinson and Company
NJ, US
IPC
G01N 21/03
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