05709854 is referenced by 221 patents and cites 78 patents.

A cell-polymeric solution is injected into an animal where the polymer crosslinks to form a polymeric hydrogel containing dispersed cells and the cells form new tissue in the animal. The polymer is biodegradable and is a natural polymer such as alginate or a synthetic polymer. The cells are chondrocytes, osteoblasts, muscle cells, fibroblasts or cells acting primarily to synthesize, secret or metabolize materials. Crosslinking of the polymer results from using cations or anions, altering the pH or changing the temperature. A polyion such as polyethyleneimine or polylysine can be added before injection to stabilize the polymeric hydrogel. A kit for tissue formation is provided by combining the cell-polymeric solution with a means for injecting the solution into an animal.

Title
Tissue formation by injecting a cell-polymeric solution that gels in vivo
Application Number
8/56140
Publication Number
5709854
Application Date
April 30, 1993
Publication Date
January 20, 1998
Inventor
Keith T Paige
Brookline
MA, US
Charles A Vacanti
Lexington
MA, US
Anthony Atala
Wesson
MA, US
Linda Griffith Cima
Lexington
MA, US
Agent
Arnall Golden & Gregory
Assignee
Massachusetts Institute of Technology
MA, US
IPC
C12N 11/04
C12N 11/10
C12N 5/06
C12N 5/08
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