05696314 is referenced by 170 patents and cites 16 patents.

An electrochemical sensor is designed for use in detecting a concentration of an analyte in a sample solution. The sensor to have a longer useful lifetime, improved stability and improved response time for single or multiple use applications. In one aspect, some of these goals are achieved by providing the multilayer enzyme electrode membrane having both nonporous and microporous layers. The microporous layer is formed from a polymer binder, a polymer powder, a surfactant and a mineral powder. In another aspect, certain of these goals are achieved by the use of a layer comprised of an enzyme disposed within a polymer matrix. In certain embodiments, the multilayer enzyme electrode membrane includes an outermost layer which is capable of dissolving in the sample solution such that the interface between the multilayer enzyme electrode membrane and the sample solution is continually renewed. The sensor provides an electrical current output proportional to the reagent chemical interaction of the analyte with an immobilized enzyme layer during exposure of the sensor in such solution.

Title
Multilayer enzyme electrode membranes and methods of making same
Application Number
8/679089
Publication Number
5696314
Application Date
July 12, 1996
Publication Date
December 9, 1997
Inventor
Peter Edelman
Franklin
MA, US
Kimiya Takeshita
Sanda
JP
Josef Brown
Salem
NH, US
Michael Flanagan
Needham
MA, US
James Flaherty
Attleboro
MA, US
Mark Boden
Millbury
MA, US
Susan Holbert
Holliston
MA, US
Katarina Tkacik
Concord
MA, US
Bonnie Dalzell
Sherborn
MA, US
Robert McCaffrey
Franklin
MA, US
Agent
Robert P Blackburn
Stanley Sacks
Arthur S Morgenstern
Assignee
Chiron Diagnostics Corporation
MA, US
IPC
H01L 29/78
G01N 33/66
G01N 33/535
G01N 27/26
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