05693343 is referenced by 8 patents and cites 2 patents.

In a solvent extraction process for preparing microspheres of a biodegrade polymer, the improvement comprising: preparing a homogenized antigen-sucrose matrix and adding a solvent to the sucrose-antigen matrix to form a solution; preparing a solution of a biodegradable polymer by adding a solvent to the polymer; adding the biodegradable polymer solution to the antigen-sucrose solution; adding an oil to the polymer-sucrose-antigen solution to form an emulsion having a controlled viscosity that corresponds to a predetermined average particle size of distributions of microspheres of biodegradable polymers; centrifuging the emulsion of controlled viscosity and removing the supernatant to obtain microspheres of a predetermined range of particle size distributions of from about 0.5 to about 7.0 micrometers.

An immunostimulating composition comprising an encapsulating-microsphere of the biodegradable polymer has an average particle size distribution such that the majority of the microspheres will be taken up by the villous epithelium section of the intestines of a mammalian subject when administered as a vaccine against diseases caused by enteropathogenic organisms.

Title
Microparticle carriers of maximal uptake capacity by both M cells and non-M cells
Application Number
590308
Publication Number
5693343
Application Date
May 16, 1994
Publication Date
December 2, 1997
Inventor
Curt Thies
Ballwin
MO, US
Egar C Boedeker
Chevy Chase
MD, US
William R Brown
Denver
CO, US
John E van Hamont
Fort Meade
MD, US
Robert H Reid
Kensington
MD, US
Agent
Werten F W Bellamy
Assignee
The United States of America represented by the Secretary of the Army
DC, US
IPC
A61K 47/30
A61K 9/50
A61K 9/16
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