05656493 is referenced by 315 patents and cites 53 patents.

There is disclosed herein a machine for performing nucleic acid amplification under computer control. The machine utilizes any one of a number of heating and cooling systems under control of a host computer which directs the heating and cooling systems to heat and cool a reaction-chamber-containing heat exchanger at appropriate times in the process. The reaction chambers are pre-loaded with the nucleic acid(s) to be amplified, a thermostable enzyme to catalyze polymerization, specific oligonucleotide primers, and four different nucleotide triphosphates. Also disclosed is the process for the amplification chain reaction implemented by the machine, which utilizes a thermostable enzyme.

Title
System for automated performance of the polymerase chain reaction
Application Number
716975
Publication Number
5656493
Application Date
February 18, 1994
Publication Date
August 12, 1997
Inventor
Joseph T Widunas
Freemont
CA, US
Louis M Mezei
Madison
WI, US
Timothy J Wennberg
Mariposa
CA, US
Richard A Leath
Berkley
CA, US
Larry Johnson
San Jose
CA, US
Kary B Mullis
LaJolla
CA, US
Agent
Richard P Ferrara
William J Hone
Assignee
The Perkin Elmer Corporation
CT, US
IPC
G05B 19/10
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