05635358 is referenced by 398 patents and cites 22 patents.

Disclosed are devices and methods for analyzing a fluid cell containing sample. The devices are composed of a solid substrate, microfabricated to define at least one sample inlet port and a mesoscale flow system. The mesoscale flow system includes a sample flow channel, extending from the inlet port, and a cell handling region for treating cells disposed in fluid communication with the flow channel. The devices may further include a component for inducing flow of cells in the sample through the flow system. In one embodiment, the cell-handling region may include a cell lysis component to enable the lysis of cells in the sample, prior to, e.g., the detection of an intracellular component in the cell sample. In another embodiment, the cell handling region may have a cell capture region, with binding sites which reversibly bind to a specific population of cells in the cell sample, to permit the isolation of the specific cell population from the sample. The devices can be utilized in a wide range of automated sensitive and rapid tests for the analysis of a fluid cell containing sample.

Title
Fluid handling methods for use in mesoscale analytical devices
Application Number
877536
Publication Number
5635358
Application Date
February 14, 1994
Publication Date
June 3, 1997
Inventor
Jay N Zemel
Jenkintown
PA, US
Larry J Kricka
Berwyn
PA, US
Peter Wilding
Paoli
PA, US
Agent
Dann Dorfman Herrell and Skillman
Assignee
Trustees of the University of Pennsylvania
PA, US
IPC
G01N 33/558
G01N 33/543
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