05604101 is referenced by 46 patents and cites 10 patents.

A disposable reaction vessel for performing nucleic acid amplification assay. The disposable reaction vessel has a penetrable cap that can be penetrated by an automated pipettor to aspirate a portion of an amplified reaction product. The disposable reaction vessel contains the reagents necessary to perform a nucleic acid amplification assay. A patient specimen is added to the unit dose reagents in the disposable reaction vessel and the penetrable cap is closed. The disposable reaction vessel containing the reaction mixture and the specimen undergoes amplification, typically by placing it in a thermal cycler. After amplification the intact disposable reaction vessel is transferred to an automated analyzer where an automated pipettor penetrates the closure membrane and aspirates a portion of the amplified sample for further processing, without removal of the reaction vessel cap. This avoids the generation of potentially contaminating aerosols or droplets.

Title
Method of minimizing contamination in amplification reactions using a reaction tube with a penetrable membrane
Application Number
140632
Publication Number
5604101
Application Date
November 17, 1995
Publication Date
February 18, 1997
Inventor
Thomas F Zurek
River Forest
IL, US
Curtis J Pepe
McHenry
IL, US
Helen H Lee
Lake Forest
IL, US
A David Hofferbert
Grafton
WI, US
Kathleen A Hanley
Gurnee
IL, US
Agent
Paul D Yasger
Thomas D Brainard
Assignee
Abbott Laboratories
IL, US
IPC
C12P 19/34
C12Q 1/68
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