05604097 is referenced by 314 patents and cites 16 patents.

The invention provides a method of tracking, identifying, and/or sorting classes or subpopulations of molecules by the use of oligonucleotide tags. Oligonucleotide tags of the invention each consist of a plurality of subunits 3 to 6 nucleotides in length selected from a minimally cross-hybridizing set. A subunit of a minimally cross-hybridizing set forms a duplex or triplex having two or more mismatches with the complement of any other subunit of the same set. The number of oligonucleotide tags available in a particular embodiment depends on the number of subunits per tag and on the length of the subunit. An important aspect of the invention is the use of the oligonucleotide tags for sorting polynucleotides by specifically hybridizing tags attached to the polynucleotides to their complements on solid phase supports. This embodiment provides a readily automated system for manipulating and sorting polynucleotides, particularly useful in large-scale parallel operations, such as large-scale DNA sequencing, mRNA fingerprinting, and the like, wherein many target polynucleotides or many segments of a single target polynucleotide are sequenced simultaneously.

Title
Methods for sorting polynucleotides using oligonucleotide tags
Application Number
322348
Publication Number
5604097
Application Date
December 19, 1994
Publication Date
February 18, 1997
Inventor
Sydney Brenner
Cambridge
GB
Agent
Stephen C Macevicz
Assignee
Spectragen
CA, US
IPC
C07H 21/00
C12N 15/10
C12Q 1/68
View Original Source