The invention was developed in order to effectively crush and disperse various samples easily and efficiently without using any expensive device. By laminating a polyethylene film 3, a net member 7, a net member 9 and a polyethylene film 5 in sequence and welding these members to one another at weld portions 13a and 13b, a bag member 15 is formed. Further by forming a fastener 17 in the vicinity of an opening 15a, a crushing and dispersing bag 1 is completed. After a sample and a dispersing medium are introduced through the opening 15a, the bag is sealed with the fastener 17. Subsequently, when the crushing and dispersing bag 1 is squeezed, the sample is ground by the file-like irregularities formed by the net members 7 and 9 on the inner surface of the bag 1.