05554339 is referenced by 303 patents and cites 9 patents.

An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments. The present invention also discloses a novel method for the electrochemical detection of particular analyte species of biological and physiological significance using an substrate/label signal generating pair which produces a change in the concentration of electroactive species selected from the group consisting of dioxygen and hydrogen peroxide.

Title
Process for the manufacture of wholly microfabricated biosensors
Application Number
270171
Publication Number
5554339
Application Date
August 19, 1993
Publication Date
September 10, 1996
Inventor
Henry J Wieck
Plainsboro
NJ, US
Paul Van Der Werf
Princeton Junction
NJ, US
Nicolaas Smit
Hightstown
NJ, US
Sylvia Piznik
Jackson
NJ, US
Randall M Mier
Morrisvile
PA, US
Imants R Lauks
Yardley
PA, US
Graham Davis
Plainsboro
NJ, US
Stephen N Cozzette
Nepean
CA
Agent
Pennie & Edmonds
Assignee
i Stat Corporation
NJ, US
IPC
C12M 1/00
G01N 30/00
G01N 21/01
G01N 21/00
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