05545522 is referenced by 183 patents and cites 2 patents.

This invention relates to the use of promoters for ribonucleic acid amplification and other genetic manipulations. Processes are provided wherein complementary deoxyribonucleic acid (cDNA) is synthesized from a ribonucleic acid (RNA) sequence using a complementary primer linked to an RNA polymerase promoter region complement and then anti-sense RNA (aRNA) is transcribed from the cDNA by introducing an RNA polymerase capable of binding to the promoter region. Additional processes using the resulting aRNA are also described.

Title
Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
Application Number
411370
Publication Number
5545522
Application Date
October 5, 1992
Publication Date
August 13, 1996
Inventor
James H Eberwine
1025 Clinton Ave. #202, Philadelphia, 19107
PA, US
Jack D Barchas
1900 Rittenhouse Sq. #18A, Philadelphia, 19103
PA, US
Mark E Von Zastrow
11570 Buena Vista Dr., Los Altos, 94022
CA, US
Russell N Van Gelder
2200 Divot Dr., Crystal Lake, 63131
MD, US
Agent
Tracy J Dunn
IPC
C12Q 1/68
C12P 19/34
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