05516532 is referenced by 228 patents and cites 3 patents.

Ground bone or cartilage particles are demineralized by extraction with a low ionic strength buffer such as 20 mM HEPES containing a chelating agent and protease inhibitors, then extracted with an acidic solution such as 0.3M citric acid, pH 4.0, containing protease inhibitors. The extracted material generally contains less than 2% by weight phosphate and less than 100 mM calcium. The phosphate content can be further reduced by treatment of the matrix with acid phosphatase, which removes residual organic phosphate. The material is useful in a method of treatment of vesicouretal reflux and other disorders where a bulking agent is effective is correcting the defect.

Title
Injectable non-immunogenic cartilage and bone preparation
Application Number
8/286273
Publication Number
5516532
Application Date
August 5, 1994
Publication Date
May 14, 1996
Inventor
Samy Ashkar
Boston
MA, US
Anthony Atala
Weston
MA, US
Agent
Arnall Golden & Gregory
Assignee
Children s Medical Center Corporation
MA, US
IPC
A61K 35/32
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