05514555 is referenced by 54 patents and cites 10 patents.

The present invention relates to a novel assay for lymphocyte chemotaxis. The assay is transendothelial assay using endothelial cells cultured on microporous filters. Lymphocyte transmigration through the filter toward a suspected chemoattractant is measured. Apparatuses for carrying out the assay are also provided. The apparatuses and methods of the present invention can be used for the identification of inhibitors (e.g., antagonists) or promoters (chemoattractants) of the adhesion receptor-mediated migration of leukocytes through the endothelium (extravasation). Such inhibitors and promoters respectively inhibit and promote the inflammatory response, and thus have therapeutic utilities. The inhibitors and promoters are identified by detecting their abilities to respectively inhibit or promote the chemotaxis of lymphocytes in the assay of the invention. The assay of the invention also has diagnostic utilities for detecting a disease or disorder involving a defect in lymphocyte chemotaxis. In a specific embodiment, the invention provides a novel lymphocyte chemoattractant, termed LCA, of molecular weight of about 14,500.+-.3,000 daltons. Derivatives and analogs of LCA, and antibodies and antibody fragments thereto are also provided. The invention also relates to therapeutic uses and compositions related to the foregoing.

Title
Assays and therapeutic methods based on lymphocyte chemoattractants
Application Number
8/30764
Publication Number
5514555
Application Date
March 12, 1993
Publication Date
May 7, 1996
Inventor
Michelle W Carr
Boston
MA, US
Stephen J Roth
Brookline
MA, US
Timothy A Springer
Chestnut Hill
MA, US
Agent
Pennie & Edmonds
Assignee
Center for Blood Research
MA, US
IPC
G01N 33/567
C12Q 1/02
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