05494810 is referenced by 363 patents and cites 9 patents.

The present invention relates to the cloning of the gene of a thermophilic DNA ligase, from Thermus aquaticus strain HB8, and the use of this ligase in a ligase chain reaction (LCR) assay for the detection of specific sequences of nucleotides in a variety of nucleic acid samples, and more particularly in those samples containing a DNA sequence characterized by a difference in the nucleic acid sequence from a standard sequence including single nucleic acid base pair changes, deletions, insertions or translocations.

Title
Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
Application Number
518447
Publication Number
5494810
Application Date
November 22, 1994
Publication Date
February 27, 1996
Inventor
Leroy Hood
Seattle
WA, US
Robert J Kaiser Jr
Seattle
WA, US
Deborah Nickerson
Seattle
WA, US
John Zebala
New York
NY, US
Francis Barany
New York
NY, US
Agent
Nixon Hargrave Devans & Doyle
Assignee
Cornell Research Foundation
NY, US
IPC
C12P 19/34
C12Q 1/25
C12Q 1/68
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