05486335 is referenced by 398 patents and cites 23 patents.

Disclosed are devices and methods for detecting the presence of a preselected analyte in a fluid sample. The invention provides a device comprising a solid substrate, typically on the order of a few millimeters thick and approximately a 0.2 to 2.0 centimeters square, microfabricated to define a sample inlet port and a mesoscale flow system. A sample is passed through the mesoscale flow system, and the restriction or blockage of flow through the flow system is detected as a positive indication of the presence of the analyte. The mesoscale flow system includes in one embodiment a primary sample flow channel extending from the inlet port, and a fractal region, in fluid communication with the flow channel, comprising bifurcations leading to plural secondary flow channels. The device may be adapted for operation in conjunction with a pump, for example, to induce flow of a sample through the flow system. A detector may also be provided for detecting analyte induced changes in flow properties in the mesoscale flow system. The devices of the invention may be used in a wide range of applications, including the detection of cells or macromolecules, such as viruses.

Title
Analysis based on flow restriction
Application Number
877701
Publication Number
5486335
Application Date
April 24, 1995
Publication Date
January 23, 1996
Inventor
Larry J Kricka
Berwyn
PA, US
Peter Wilding
Paoli
PA, US
Agent
Dann Dorfman Herrell and Skillman
Assignee
Trustees of the University of Pennsylvania
PA, US
IPC
G01N 21/00
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