05472881 is referenced by 237 patents and cites 7 patents.

A method for adsorbing nucleic acids to a surface of a gold substrate for analyzing the structure of the nucleic acid includes the steps of thiolating the nucleic acid by substituting at least one non-bridging internucleotide oxygen of each phosphodiester moiety with sulfur, depositing the thiolated nucleic acid on the gold surface, and subjecting the nucleic acid to analysis for determining the atomic or molecular structure thereof. The gold surface is prepared by subjecting a gold single crystal to mechanical polishing, electropolishing, cleaning by cycles of Ar.sup.+ sputtering and annealing under vacuum until no contamination is detected by Auger electron spectroscopy, and flame annealing and quenching in methanol. Gold films deposited on atomically flat substrates, such as mica, can also be used. The thiolated DNA is deposited on the gold surface for a sufficient time for covalent bonds to form between the sulfur and the gold. Scanning tunneling microscopy, atomic force microscopy, angle-dependent x-ray photoelectron spectroscopy, and Auger electron spectroscopy are used to analyze the nucleic acid structure.

Title
Thiol labeling of DNA for attachment to gold surfaces
Application Number
974564
Publication Number
5472881
Application Date
March 21, 1994
Publication Date
December 5, 1995
Inventor
Carol E Rabke Clemmer
Rochester
NY, US
Thomas P Beebe
Salt Lake City
UT, US
Agent
Thorpe North & Western
Assignee
University of Utah Research Foundation
UT, US
IPC
G01N 33/00
C12Q 1/68
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