05436128 is referenced by 171 patents and cites 10 patents.

Transcription based assays that identify extracellular signals that modulate the activity of cell surface proteins are provided. Extracellular signals are identified by measuring the amount of transcription of a reporter gene in a recombinant cell that expresses the cell surface protein and contains DNA encoding the reporter gene under the transcriptional control of a promoter that is regulated, directly or indirectly, by the cell surface protein. The assays, provide a means for identifying potential pharmaceutical compounds that can be used to treat disease by virtue of their agonistic or antagonistic effects on the cell surface protein. Recombinant cells that express cell surface receptors and that contain reporter gene constructs that include transcriptional regulatory elements that are responsive to the activity of the cell surface receptors are also provided.

Title
Assay methods and compositions for detecting and evaluating the intracellular transduction of an extracellular signal
Application Number
563751
Publication Number
5436128
Application Date
January 27, 1993
Publication Date
July 25, 1995
Inventor
Paul Brust
San Diego
CA, US
Michael M Harpold
El Cajon
CA, US
Agent
Brown Martin Haller & McClain
Assignee
Salk Institute Biotechnology Industrial Associates
CA, US
IPC
G01N 33/00
G01N 33/48
C12N 15/63
C12Q 1/68
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