05415839 is referenced by 235 patents and cites 8 patents.

Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a two-tier thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then mated with a detection chamber to form the reaction detection unit. A first heating element of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber by the second heating element and amplified target nucleic acid is immobilized on a support in the detection chamber. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target.

Title
Apparatus and method for amplifying and detecting target nucleic acids
Application Number
8/140731
Publication Number
5415839
Application Date
October 21, 1993
Publication Date
May 16, 1995
Inventor
John J Kotlarik
Vernon Hills
IL, US
Julian Gordon
Lake Bluff
IL, US
Stanley R Bouma
Grayslake
IL, US
Peter Zaun
Libertyville
IL, US
Agent
Thomas D Brainard
Assignee
Abbott Laboratories
IL, US
IPC
C12M 1/40
C12P 17/34
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