05372945 is referenced by 106 patents and cites 24 patents.

Methods and apparatus for collecting and processing tissue to produce an endothelial cell product having a vessel for rinsing, draining, digesting and isolating tissue. The vessel has a rinsing and digesting chamber for containing tissue during processing. An inlet in the rinsing and digesting chamber allows entry of rinsing solution and tissue from a liposuction device. A waste chamber in fluid communication with the rinsing and digesting chamber preferably connects with a vacuum source. An isolation chamber is separated from the rinsing and digesting chamber by a screen. An ampule in fluid communication with the isolation chamber includes a pair of ports controlled by valve devices to be selectively in fluid communication with the isolation chamber. After processing, the ampule isolates a pellet of endothelial cells and the valve devices permit the pellet to be in fluid communication with the ports. The method includes providing the vessel, introducing tissue to be processed, orienting the vessel to screen the tissue, introducing an enzyme and agitating to digest the tissue, centrifuging the vessel to transfer the cells from the digested tissue, and isolating the cells for retrieval.

Title
Device and method for collecting and processing fat tissue and procuring microvessel endothelial cells to produce endothelial cell product
Application Number
742086
Publication Number
5372945
Application Date
July 31, 1992
Publication Date
December 13, 1994
Inventor
Joseph A DiPisa Jr
84 Maryann La., Wyckoff, 07481
NJ, US
Stuart K Williams
5181 N. Circulo Sobrio, Tucson, 85718
AZ, US
Bruce E Jarrell
5700 N. Via Elena, Tucson, 85718
AZ, US
Alfred W Prais
245 Awosting Rd., Hewitt, 07421
NJ, US
Paul G Alchas
29 Ponds Cir., Wayne, 07470
NJ, US
Agent
Woodcock Washburn Kurtz Mackiewicz & Norris
IPC
C12M 1/40
C12S 3/00
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