05355215 is referenced by 163 patents and cites 18 patents.

Measurement of the fluorescence of a layer of cells, disposed in a well, with supernatant liquid thereabove, is greatly enhanced in sensitivity by illuminating the cell layer with a beam of light incident thereupon at a first angle and detecting fluorescence emitted by the cells with a detector which views the illuminated cells at a second angle, wherein at least one of the first or second angles is oblique to the cell layer. By so controlling the geometry of the system, the contribution to background fluorescence by the supernatant liquid is greatly minimized. Sensitivity of the technique is further enhanced by restricting the portion of the illuminated cell layer which is viewed by the detector. The technique may be applied to an apparatus for rapidly scanning the fluorescence of a plurality of samples in a multiple well plate.

Title
Method and apparatus for quantitative fluorescence measurements
Application Number
7/953898
Publication Number
5355215
Application Date
September 30, 1992
Publication Date
October 11, 1994
Inventor
Brad Neagle
Ann Arbor
MI, US
Kirk Schroeder
Ann Arbor
MI, US
Agent
Krass & Young
Assignee
Environmental Research Institute of Michigan
MI, US
IPC
G01N 21/64
G01J 3/30
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