05352348 is referenced by 371 patents and cites 22 patents.

Disclosed is a method of assaying a high concentration of a substance in a liquid sample with a polarographic cell. The cell contains an electrode assembly that includes a reference electrode and a hydrogen peroxide sensor electrode having a laminated membrane covering the liquid sample contacting face of the sensor electrode. The laminated membrane includes an outer membrane permeable to the substance and oxygen, an inner membrane permeable to hydrogen peroxide and located adjacent the face of the sensor electrode, and an enzyme layer between the inner and outer membrane; the enzyme in the enzyme layer can oxidize the substance to generate hydrogen peroxide. The method includes contacting the outer membrane with the liquid sample; permitting the substance and oxygen in the liquid sample to pass through the outer membrane to contact the enzyme layer so that where the substance is oxidized to generate hydrogen peroxide; permitting the generated hydrogen peroxide to pass through the inner membrane to contact the sensor electrode; and ensuring that the supply of oxygen in the enzyme layer relative to the supply of glucose is sufficient to produce an equilibrium concentration of hydrogen peroxide, which generates a steady state response at the sensor electrode proportional to the concentration of the substance in the liquid sample.

Title
Method of using enzyme electrode
Application Number
36495
Publication Number
5352348
Application Date
November 3, 1992
Publication Date
October 4, 1994
Inventor
Handani Winarta
Waltham
MA, US
Chung Chang Young
Weston
MA, US
Agent
Fish & Richardson
Assignee
Nova Biomedical Corporation
MA, US
IPC
G01N 27/40
View Original Source