05342790 is referenced by 66 patents and cites 5 patents.

A patient's health is diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more groups of particles such as lyposomes or plastic beads of different densities for each group. Each group of density-defined particles carries antigens or antibodies which are specific to a complement antigen or antibody which may be in the blood sample being tested, and which are indicative of the patient's health. A label-tagged antibody which is specific to all bound antibody/antigen couples is added to the blood sample so as to form labeled antibody+antigen-antibody complexes (AAAC) in the blood sample. Upon centrifugation, the complexed particles will settle out in different areas in the tube according to the respective density of the particles, and the degree of label emission of the particle layers can enable qualitative or quantitative analyses of the blood sample to be made. Unbound labeled antibodies will be washed away from the complexed layers by the washing action of the descending blood cells during the centrifugation step. Unbound labeled antibodies will thus not interfere with the analysis.

Title
Apparatus for indirect fluorescent assay of blood samples
Application Number
7/969379
Publication Number
5342790
Application Date
October 30, 1992
Publication Date
August 30, 1994
Inventor
Diether J Recktenwald
Cupertino
CA, US
Thomas J Mercolino
Stockton
NJ, US
Leon W M M Terstappen
Palo Alto
CA, US
Stephen C Wardlaw
Old Saybrook
CT, US
Robert A Levine
Guilford
CT, US
Agent
William W Jones
Assignee
Becton Dickinson and Company
NJ, US
IPC
G01N 33/536
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