05258498 is referenced by 365 patents and cites 23 patents.

Disclosed are a family of synthetic proteins having binding affinity for a preselected antigen, and multifunctional proteins having such affinity. The proteins are characterized by one or more sequences of amino acids constituting a region which behaves as a biosynthetic antibody binding site (BABS) The sites comprise V.sub.H -V.sub.L or V.sub.L -V.sub.H -like single chains wherein the V.sub.H and V.sub.L -like sequences are attached by a polypeptide linker, or individual V.sub.H or V.sub.L -like domains. The binding domains comprise linked CDR and FR regions, which may be derived from separate immunoglobulins. The proteins may also include other polypeptide sequences which function, e.g., as an enzyme, toxin, binding site, or site for attachment to an immobilization media or radioactive atom. Methods are disclosed for producing the proteins, for designing BABS having any specificity that can be elicited by in vivo generation of antibody for producing analogs thereof, and for producing multifunctional synthetic proteins which are self-targeted by virtue of their binding site region.

Title
Polypeptide linkers for production of biosynthetic proteins
Application Number
52800
Publication Number
5258498
Application Date
October 1, 1992
Publication Date
November 2, 1993
Inventor
Hermann Oppermann
Medway
MA, US
James S Huston
Newton
MA, US
Agent
Testa Hurwitz & Thibeault
Assignee
Creative BioMolecules
MA, US
IPC
C12P 21/00
C12N 15/09
C07K 15/00
View Original Source