05252743 is referenced by 295 patents and cites 21 patents.

Methods and compositions are described for immobilizing anti-ligands, such as antibodies or antigens, hormones or hormone receptors, oligonucleotides, and polysaccharides on surfaces of solid substrates for various uses. The methods provide surfaces covered with caged binding members which comprise protecting groups capable of being removed upon application of a suitable energy source. Spatially addressed irradiation of predefined regions on the surface permits immobilization of anti-ligands at the activated regions on the surface. Cycles of irradiation on different regions of the surface and immobilization of different anti-ligands allows formation of an immobilized matrix of anti-ligands at defined sites on the surface. The immobilized matrix of anti-ligands permits simultaneous screenings of a liquid sample for ligands having high affinities for certain anti-ligands of the matrix. A preferred embodiment of the invention involves attaching photoactivatable biotin derivatives to a surface. Photolytic activation of the biotin derivatives forms biotin analogs having strong binding affinity for avidin. Biotinylated anti-ligands can be immobilized on activated regions of the surface previously treated with avidin.

Title
Spatially-addressable immobilization of anti-ligands on surfaces
Application Number
435316
Publication Number
5252743
Application Date
November 13, 1990
Publication Date
October 12, 1993
Inventor
Steven A Sundberg
San Francisco
CA, US
Christopher P Holmes
Sunnyvale
CA, US
Lubert Stryer
Stanford
CA, US
Michael C Pirrung
Durham
NC, US
Ronald W Barrett
Sunnyvale
CA, US
Agent
Karen B Dow
Jeffrey K Weaver
Kevin R Kaster
Assignee
Affymax Technologies
NL
IPC
C07D 235/02
C07D 491/048
C07D 495/04
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