05246867 is referenced by 390 patents and cites 3 patents.

A method for measuring the concentration of a saccharide, conjugated saccharide or polysaccharide of interest using luminescent lifetimes and energy transfer in which an energy transfer donor-acceptor pair is added to a sample to be analyzed, the donor of the donor-acceptor pair being photoluminescent. The acceptor is bound to a carrier, while the donor and any saccharide, conjugated saccharide or polysaccharide of interest present in the sample compete for binding sites on the carrier. The sample is irradiated and the resultant emission detected. Energy transfer occurs between the donors and the acceptors, which produces a detectable lifetime change of the fIuorescence of the donor. The lifetime change is reduced or even eliminated by the competitive binding of a saccharide, conjugated saccharide or polysaccharide of interest to the donor. By measuring the apparent luminescent lifetime, the amount of a saccharide, conjugated saccharide or polysaccharide of interest in the sample can be determined.

Title
Determination and quantification of saccharides by luminescence lifetimes and energy transfer
Application Number
7/822382
Publication Number
5246867
Application Date
January 17, 1992
Publication Date
September 21, 1993
Inventor
Peter A Koen
Hillsborough
NJ, US
Badri P Maliwal
Baltimore
MD, US
Joseph R Lakowicz
Columbia
MD, US
Agent
Wegner Cantor Mueller & Player
Assignee
Becton Dickinson & Company
NJ, US
University of Maryland at Baltimore
MD, US
IPC
G01N 33/66
G01N 21/64
View Original Source