05244630 is referenced by 66 patents and cites 6 patents.

A disposable device suitable for performing automated solid-phase diagnostic assays which employs microparticles to complex an analyte and where the microparticle complex becomes retained and immobilized on a fibrous matrix such that the presence of analyte on the microparticles can be detached by optical means. A device is disclosed having a plurality of well pairs comprising a sample well for receiving a sample and reagents for forming a reaction mixture, a read well comprising (a) an entrance port and holder for receiving and holding a quantity of sample and assay reagents, (b) a fibrous matrix for retaining and immobilizing microparticle/analyte complexes for detection, said fibrous matrix positioned below said holder, and having an average spatial separation of fibers greater than the average diameter of said microparticles, (c) absorbent material positioned below said fibrous matrix for assisting the flow of sample and assay reagents through said fibrous matrix, (d) walls or ribs for creating a substantially light-tight real surrounding the wells, (e) a vent for venting air entrapped in the absorbent material and which is displaced by fluids absorbed therein, and (f) a passage between the sample well and the read well whereby sample and reaction mixtures can be transferred and washed from said sample well into said read well without being contacted by any apparatus.

Title
Device for performing solid-phase diagnostic assay
Application Number
184726
Publication Number
5244630
Application Date
October 23, 1989
Publication Date
September 14, 1993
Inventor
Thomas F Zurek
River Forest
IL, US
Denise G Huff
Prospect Heights
IL, US
Charles F Hanna
Libertyville
IL, US
Omar S Khalil
Libertyville
IL, US
Agent
Daniel W Collins
Priscilla E Porembski
Frank S Ungemach
Assignee
Abbott Laboratories
IL, US
IPC
G01N 33/543
G01N 1/28
View Original Source