05223409 is referenced by 1672 patents and cites 34 patents.

In order to obtain a novel binding protein against a chosen target, DNA molecules, each encoding a protein comprising one of a family of similar potential binding domains and a structural signal calling for the display of the protein on the outer surface of a chosen bacterial cell, bacterial spore or phage (genetic package) are introduced into a genetic package. The protein is expressed and the potential binding domain is displayed on the outer surface of the package. The cells or viruses bearing the binding domains which recognize the target molecule are isolated and amplified. The successful binding domains are then characterized. One or more of these successful binding domains is used as a model for the design of a new family of potential binding domains, and the process is repeated until a novel binding domain having a desired affinity for the target molecule is obtained. In one embodiment, the first family of potential binding domains is related to bovine pancreatic trypsin inhibitor, the genetic package is M13 phage, and the protein includes the outer surface transport signal of the M13 gene III protein.

Title
Directed evolution of novel binding proteins
Application Number
240160
Publication Number
5223409
Application Date
March 1, 1991
Publication Date
June 29, 1993
Inventor
Rachel B Kent
Boxborough
MA, US
Arthur C Ley
Newton
MA, US
William Markland
Milford
MA, US
Bruce L Roberts
Milford
MA, US
Sonia K Guterman
Belmont
MA, US
Robert C Ladner
Ijamsville
MD, US
Agent
Iver P Cooper
Assignee
Protein Engineering
MA, US
IPC
C12N 15/63
C12N 15/62
C12N 15/09
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