The invention provides a nonenzymatic method useful for the semiquantitative determination of glucose, a test composition and test device. Glucose concentration in an aqueous test sample can be determined by preparing a test solution by contacting an aqueous test sample and a dihydroxide component, at an initial pH above 6.5, capable of forming a complex with glucose which complex formation releases a proton, and determining the final pH of the test solution. The invention also provides a self-indicating device format which allows the determination of glucose concentration by merely counting the number of pads which have changed color. No comparison to a color chart is required. The glucose test of the present invention is free of the interferences found in enzymatic glucose tests.