05185243 is referenced by 163 patents and cites 13 patents.

A method is disclosed for detecting the presence of a target nucleotide sequence in a polynucleotide. The method comprises hybridizing a first nucleotide sequence and a second nucleotide sequence to non-contiguous portions of a target nucleotide sequence, covalently attaching the first and second sequences when they are hybridized to the target sequence, and determining the presence of covalently attached first and second sequences. The presence of the covalently attached first and second sequences is related to the presence of the target nucleotide sequence. The invention may be applied to target nucleotide sequences in DNA or RNA. Specific target nucleotide sequences of interest will frequently be characteristic of particular microorganisms, viruses, viroids, or genetic characteristics, including genetic abnormalities.

Title
Method for detection of specific nucleic acid sequences
Application Number
7/236967
Publication Number
5185243
Application Date
August 25, 1988
Publication Date
February 9, 1993
Inventor
Paul D Stull
Mountain View
CA, US
Thomas C Goodman
Mountain View
CA, US
Edwin F Ullman
Atherton
CA, US
Agent
Mark L Bosse
Theodore J Leitereg
Assignee
Syntex
CA, US
IPC
C01H 15/12
G01N 33/566
C12P 19/34
C12Q 1/68
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