05160490 is referenced by 88 patents and cites 36 patents.

The present invention relates to a three-dimensional cell culture system which can be used to culture a variety of different cells and tissues in vitro for prolonged periods of time. In accordance with the invention, cells derived from a desired tissue are inoculated and grown on a pre-established stromal support matrix. The stromal support matrix comprises stromal cells, such as fibroblasts actively growing on a three-dimensional matrix. Stromal cells may also include other cells found in loose connective tissue such as endothelial cells, macrophages/monocytes, adipocytes, pericytes, reticular cells found in bone marrow stroma, etc. The stromal matrix provides the support, growth factors, and regulatory factors necessary to sustain long-term active proliferation of cells in culture. When grown in this three-dimensional system, the proliferating cells mature and segregate properly to form components of adult tissues analogous to counterparts found in vivo.

Title
Three-dimensional cell and tissue culture apparatus
Application Number
853569
Publication Number
5160490
Application Date
February 21, 1991
Publication Date
November 3, 1992
Inventor
Brian A Naughton
Groton
VT, US
Gail K Naughton
Groton
VT, US
Agent
Pennie & Edmonds
Assignee
Marrow Tech Incorporated
CA, US
IPC
C12N 5/06
C12Q 1/18
C12M 1/00
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