05096815 is referenced by 249 patents and cites 22 patents.

Novel DNA-binding proteins, especially repressors of gene expression, are obtained by variegation of genes encoding known binding protein and selection for proteins binding the desired target DNA sequence. A novel selection vector is used to reduce artifacts. Heterooligimeric proteins which bind to a target DNA sequence which need not be palindromic are obtained by a variety of methods, e.g., variegation to obtain proteins binding symmetrized forms of the half-targets and heterodimerization to obtain a protein binding the entire asymmetric target.

Title
Generation and selection of novel DNA-binding proteins and polypeptides
Application Number
7/293980
Publication Number
5096815
Application Date
January 6, 1989
Publication Date
March 17, 1992
Inventor
Arthur C Ley
Newton
MA, US
Rachel B Kent
Wilmington
MA, US
Sonia K Guterman
Belmont
MA, US
Robert C Ladner
Ijamsville
MD, US
Agent
Iver P Cooper
Assignee
Protein Engineering Corporation
MA, US
IPC
C12P 21/00
C12N 15/09
C12N 15/63
View Original Source